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The MV-1 is an embedded on/off valve used to automate microfluidic functions such as priming, mixing, and timing of fluidic events. The valve is actuated using externally supplied air pressure via a channel on the microfluidic chip. Performance benefits of the MV-1 include optical clarity, solvent resistance, fast switching times, reliable sealing, and the ability to traffic small particles without fouling. Multiple valves can be attached to a single microfluidic chip for either simultaneous or independent control.