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LNP Screening on FLO i8 PD

High-throughput LNP screening on Formulatrix® FLO i8® PD

Ready to start screening more LNP formulations?

Share your contact information and our team will reach out to discuss your liquid handler, screening goals, and next steps.

= required

Extend the FLO i8 PD to LNP formulation

Run automated microfluidic LNP formulation directly on the FLO i8 PD with the LNP Screening Array.

100+ formulations per hour

Evaluate more conditions within each screening campaign and identify promising formulations sooner.

10x faster than LNP instruments

Generate LNPs in seconds and shorten screening cycles.

<50 µL dead volume

Use valuable lipid and RNA inputs across more formulation conditions.

1 automated workflow

Keep reagent handling, LNP formulation, sample collection, and downstream transfer in one run.

Why the FLO i8 PD is well suited for LNP screening

Positive displacement pipetting gives the FLO i8 PD direct, channel-level control over liquid delivery. Total flow rate and aqueous-to-lipid flow rate ratio can be programmed within the method, allowing teams to evaluate process conditions without changing the consumable architecture.

Positive displacement control
Program liquid delivery directly at each channel for precise execution of formulation conditions.

Programmable flow conditions
Define total flow rate and aqueous-to-lipid flow rate ratio within the automation method.

Microfluidic formulation on deck
Add particle generation to the existing automation workflow without introducing a separate formulation instrument.

Performance on the FLO i8 PD

Under the tested blank LNP conditions at a 3:1 aqueous-to-lipid flow rate ratio and 1.75 mL/min total flow rate, the workflow produced sub-60 nm particles with low polydispersity and consistent particle size across the tested samples.

57.9 nm
Z-average diameter

0.12 PDI
PDI

3.28%
Z-average CV

These results establish an initial benchmark for automated LNP formulation on the FLO i8 PD and a reference point for evaluating additional lipid compositions, cargos, concentrations, and process conditions.

From reagent setup to LNPs in one automated sequence.

When formulation runs on a separate system, every transfer adds time and another point of coordination. The LNP Screening Array brings particle generation into the FLO i8 PD workflow.

1

Prepare inputs on deck.
Position aqueous and lipid inputs on the FLO i8 PD for controlled delivery.

2

Generate LNPs with the array.
Dispense through the LNP Screening Array to run parallel microfluidic formulation.

3

Collect samples for characterization.
Recover each formulated sample for sizing and downstream analysis.

A supported path to your first LNP run

Starter Kit

The LNP Screening Array Starter Kit provides the consumables, protocol, and setup components needed to establish automated microfluidic LNP formulation on the FLO i8 PD.

LNP Screening Array

  • Eight independent micromixers per array for higher-throughput formulation screening

FLO i8 PD automation protocol

  • A platform-specific method for automated reagent delivery, formulation, and sample collection

Setup components

  • Deck and sample-handling components required to establish the workflow

Parallel Fluidics and Formulatrix support method transfer, workflow setup, and initial runs.

Ready to start screening more LNP formulations?

Share your contact information and our team will reach out to discuss your liquid handler, screening goals, and next steps.

= required

Evaluate LNP formulation on your FLO i8 PD

Apply the workflow under defined formulation conditions and generate initial particle data on a platform already embedded in your automation strategy.

Ready to start screening more LNP formulations?

Share your contact information and our team will reach out to discuss your liquid handler, screening goals, and next steps.

= required

Questions about workflow fit or setup? Talk to our applications team.

Technical resources

Fact sheet
A concise overview of workflow benefits, performance, and technical specifications.

Press release
Partnership announcement and overview of the integrated workflow.

FAQ

The LNP Screening Array brings microfluidic LNP formulation directly onto the FLO i8 PD.

Each array contains 8 independent micromixers, allowing the instrument’s 8-channel head to generate 8 formulations per array within an automated liquid handling workflow.

The FLO i8 PD uses positive displacement pipetting to control liquid delivery directly at each channel.

Total flow rate and aqueous-to-lipid flow rate ratio can be programmed within the automation method, giving teams method-level control over key formulation conditions while using a standardized microfluidic array.

Under the tested blank LNP conditions at a 3:1 aqueous-to-lipid flow rate ratio and 1.75 mL/min total flow rate, the workflow produced a Z-average diameter of 57.9 nm, a PDI of 0.12, and a Z-average CV of 3.2%.

These results provide an initial benchmark for automated LNP formulation on the FLO i8 PD.

The workflow supports more than 100 formulations per hour, with 8 formulations generated per array.

Each micromixer has less than 25 µL of dead volume, helping teams evaluate more conditions from a limited supply of lipid, RNA, or other valuable inputs.

Yes. The workflow is intended to support formulation-specific evaluation across lipid composition, cargo, concentration, total flow rate, and flow rate ratio.

Results may vary by formulation, so customer-specific conditions should be evaluated during method development.

The LNP Screening Array Starter Kit is configured for the FLO i8 PD and includes the automation protocol and setup components required to establish the workflow.

Parallel Fluidics and Formulatrix support method transfer and initial setup. Final compatibility should be confirmed for your deck configuration and application requirements.

The LNP Screening Array is designed to occupy a standard deck position and connect through standard pipette tips. It does not require external tubing or a separate dedicated LNP formulation instrument.

Setup requirements should be confirmed for the specific FLO i8 PD configuration.

Yes. Teams can use the FLO i8 PD workflow to expand early screening capacity, run parallel studies, and reduce demand on dedicated systems.

Promising formulations can then move into downstream optimization or scale-up workflows as appropriate.

The workflow is designed for formulation screening and early development, where throughput, iteration speed, and efficient material use are primary requirements.

It is not positioned as a manufacturing or production-scale formulation solution.

The Starter Kit includes:

  • LNP Screening Arrays
  • FLO i8 PD automation protocol
  • Setup components
  • Support for method transfer, workflow setup, and initial runs

Request an evaluation to discuss your FLO i8 PD configuration, formulation requirements, and preferred test conditions.

Parallel Fluidics and Formulatrix can support workflow setup, initial runs, and demonstration planning.

Request an evaluation.